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Inside the Assassin Tomb beneath Santa Maria della Visitazione, if Ezio stares into the water near the first lever for about a minute, a cutscene plays showing a giant squid swimming past. Look again and a tentacle nearly slaps him.
Although he never appears on screen, the real-life assassination of Galeazzo Maria Sforza, Duke of Milan, is referenced in letters Giovanni Auditore writes to Lorenzo de' Medici, framing it as the opening spark of the game's Templar conspiracy in 1476.
Caterina Sforza, one of the game's major allies and a romantic interest for Ezio, was the real illegitimate daughter of Galeazzo Maria Sforza, the same Duke of Milan whose assassination kicks off the story.
Looting practice dummies during the missions "Knowledge Is Power" and "Hunting the Hunter" grants the player keys, leftover, dummied-out content that serves absolutely no purpose in the game.
