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Subject 16 hid twenty encoded Glyphs across the Animus recreation of Renaissance Italy, five in Florence, one in Monteriggioni, five in Tuscany, two in Romagna, and seven in Venice, each unlocking a fragment of the video known as "The Truth."
After meeting Niccolò Machiavelli, returning to the Palazzo Auditore courtyard and using Eagle Vision triggers a one-time-only vision of Ezio's entire deceased family standing around the palazzo.
When Ezio first meets his uncle Mario Auditore, Mario greets him with "It's-a-me! Mario!", a direct lift of the first line spoken by Mario in Super Mario 64.
Solving a sonar-based Glyph puzzle reveals a hidden message quoting a Hopi prophecy warning of "a container of ashes" thrown from the sky that would burn the land and boil the ocean, an omen tying into the series' looming solar-flare apocalypse.
